A dye–ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G

Poly(2-hydroxyethylmethacrylate) (pHEMA) membrane was prepared by photo-polymerization and a dye ligand (i.e., Reactive Green 5) was immobilised on the membrane surface (pHEMA-RG-5). Surface wettability and hydrophilicity of the dye–ligand immobilised membranes were investigated by static contact angle measurements. The chromatographic properties of the dye–ligand immobilized membrane for adsorption and purification of IgG are discussed using bare membrane as a control system. The non-specific adsorption of IgG was low (0.45 mg protein ml−1 membrane). The attachment of dye ligand onto the membrane significantly increased the IgG adsorption (33.75 mg protein ml−1 membrane). The maximum IgG adsorption was observed at pH 6.0. The reversible IgG adsorption on the membrane obeyed both the Freundlich and the Langmuir–Freundlich isotherm models. To test the efficiency of IgG isolation from human serum with the pHEMA-RG-5 membrane, the purity of the eluted IgG, as determined by HPLC, was 81% with a recovery of 67%.