Electroenzymatic process to overcome enzyme instabilities

• H2O2 can be produced using a gas diffusion cathode depending on the applied current.
• ttn of up to 1,150,000 was achieved for CPO using an electroenzymatic system.
• CPO is more stable than assumed.
• Novel dimensionless parameter for process design is introduced.
• Space time yields of up to 52 g L− 1 d− 1 can be reached.

The versatile enzyme chloroperoxidase was used in a reaction system, based on a gas diffusion electrode, for enzymatic chlorinations. Due to an adjusted and continuous electro-generation of the co-substrate hydrogen peroxide a ttn up to 1,150,000 for the CPO was achieved. Space time yields were dependent on the electrochemically produced H2O2 and reached up to 52 g L− 1 d− 1. The ratio of hydrogen peroxide production per added enzyme unit can be used as a dimensionless parameter for process characterization and a knowledge based process design.

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