کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4978460 1452431 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional loss of GABA transaminase (GABA-T) expressed early leaf senescence under various stress conditions in Arabidopsis thaliana
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Functional loss of GABA transaminase (GABA-T) expressed early leaf senescence under various stress conditions in Arabidopsis thaliana
چکیده انگلیسی

GABA-transaminase (GABA-T) involved in carbon and nitrogen metabolism during the plant development process via GABA shunt and GABA-T mutant, which is defective in GABA catabolism, is ideal model to examine the role of GABA-T in plant development and leaf senescence of plant. We have characterized GABA transaminase knock out mutant pop2-1 that is transition and pop2-3 which is T-DNA insertion mutant of Arabidopsis thaliana during various stress conditions.The GABA-T knockout mutant plants displayed precocious leaf senescence, which was accompanied by the assays of physiological parameters of leaf senescence during various stress conditions. Furthermore, our physiological evidence indicates that pop2-1 and pop2-3 mutations rapidly decreased the efficiency of leaf photosynthesis, chlorophyll content, GABA content, GABA-T, and glutamate decarboxylase (GAD) activity and on the other hand increases membrane ion leakage, malondialdehyde (MDA) level in stress induced leaves. However, cell viability assay by trypan blue and insitu Hydrogen peroxidation assay by 3,3-diaminobenzidine (DAB) in stress induced leaves also display that pop2-1 and pop2-3 mutant leaves show oversensitivity in response to different stress conditions as compared to wild type. These results strongly indicate that the loss-of-function of GABA transaminase gene induces early leaf senescence in Arabidopsis thaliana during various stress conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Plant Biology - Volumes 9–10, June 2017, Pages 11-22
نویسندگان
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