کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4980066 | 1453039 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Utilization of unmodified gold nanoparticles for label-free detection of mercury (II): Insight into rational design of mercury-specific oligonucleotides
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کلمات کلیدی
DLST–Hg2+–TMercury (II) ionMOPSMBSMSOLSPRHR-TEMPDIAuNPs - AuNP هاTem - این استLabel-free detection - تشخیص بدون برچسبMagnetic beads - دانه های مغناطیسیRoom temperature - دمای اتاقLocal surface plasmon resonance - رزونانس سطح پلاسمونColorimetry - رنگ سنجیHigh Resolution Transmission Electron Microscopy - میکروسکوپ الکترونی با وضوح بالاTransmission electron microscopy - میکروسکوپ الکترونی عبوریGold nanoparticles - نانوذرات طلاDynamic Light Scattering - پراکندگی نور دینامیکی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بهداشت و امنیت شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Colorimetric detection of mercury (II) with the use of DNA oligonucleotides and unmodified gold nanoparticles (AuNPs) as indicators has been extensively studied. This study provides in-depth insights into the rational design of mercury-specific oligonucleotides (MSO) in the biosensing system. The leftover bases of MSO, as a result of the formation of T-Hg2+-T base pairs, can adsorb on the AuNPs and hinder their aggregation at concentrations of salt. This phenomenon was directly verified by the changes in particle sizes characterized by dynamic light scattering for the first time. Based on these findings, we proposed a rational design for the MSO with approximately 20-fold improvement in detection sensitivity. The detection limit of the proposed assay decreased to 15Â nM with a linear working range from 50Â nM to 300Â nM for Hg2+. The cross-reactivity against eight other metal ions was negligible compared with the response to Hg2+. Considering the diverse applications of AuNPs with oligonucleotides, this study can serve as a good reference and provides important implications in sensing and DNA-directed nanoparticle assembly.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hazardous Materials - Volume 321, 5 January 2017, Pages 417-423
Journal: Journal of Hazardous Materials - Volume 321, 5 January 2017, Pages 417-423
نویسندگان
Abdul Ghaffar Memon, Xiaohong Zhou, Jinchuan Liu, Ruoyu Wang, Lanhua Liu, Bofan Yu, Miao He, Hanchang Shi,