Full Length ArticleDevelopment of a porous 3D graphene-PDMS scaffold for improved osseointegration

- Efficient fabrication method of durable and porous RGO/PDMS scaffold was developed.
- The RGO/PDMS scaffold could support growth and osteogenic differentiation of ADSCs.
- The scaffold shows potential application for better osseointegration in surgery.

Osseointegration in orthopedic surgery plays an important role for bone implantation success. Traditional treatment of implant surface aimed at improved osseointegration has limited capability for its poor performance in supporting cell growth and proliferation. Polydimethylsiloxane (PDMS) is a widely used silicon-based organic polymer material with properties that are useful in cosmetics, domestic applications and mechanical engineering. In addition, the biocompatibility of PDMS, in part due to the high solubility of oxygen, makes it an ideal material for cell-based implants. Notwithstanding its potential, a property that can inhibit PDMS bioactivity is the high hydrophobicity, limiting its use to date in tissue engineering. Here, we describe an efficient approach to produce porous, durable and cytocompatible PDMS-based 3D structures, coated with reduced graphene oxide (RGO). The RGO/PDMS scaffold has good mechanical strength and with pore sizes ranging from 10 to 600 μm. Importantly, the scaffold is able to support growth and differentiation of human adipose stem cells (ADSCs) to an osteogenic cell lineage, indicative of its potential as a transition structure of an osseointegrated implant.

Schematic illustration of the fabrication process and application for improved osseointegration of porous RGO/PDMS scaffold. [Abbreviations: NaCl, sodium chloride; EtOH, ethanol; PDMS, polydimethylsiloxane; RGO, reduced graphene oxide].128