کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4996467 1459901 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enrichment and characterization of hydrocarbon-degrading bacteria from petroleum refinery waste as potent bioaugmentation agent for in situ bioremediation
ترجمه فارسی عنوان
غنی سازی و مشخص کردن باکتری های تجزیه کننده هیدروکربن از زباله های پالایشگاهی نفت به عنوان عامل افزایش دهنده زیست محیطی برای بهبود زیست محیطی در محیط زیست
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی


- Petroleum refinery waste of Digboi refinery harbours hydrocarbonclastic bacteria.
- Isolation of superior hydrocarbon degrading bacteria achieved only by enrichment.
- Metabolic versatility of Burkholderia, Pandoraea and Enterobacter spp. explored.
- Microcosm study identified selected strains as potent bioaugmentation agent.

Intrinsic biodegradation potential of bacteria from petroleum refinery waste was investigated through isolation of cultivable strains and their characterization. Pseudomonas and Bacillus spp. populated the normal cultivable taxa while prolonged enrichment with hydrocarbons and crude oil yielded hydrocarbonoclastic bacteria of genera Burkholderia, Enterobacter, Kocuria, Pandoraea, etc. Strains isolated through enrichment showed assemblages of superior metabolic properties: utilization of aliphatic (C6-C22) and polyaromatic compounds, anaerobic growth with multiple terminal electron acceptors and higher biosurfactant production. Biodegradation of dodecane was studied thoroughly by GC-MS along with detection of gene encoding alkane hydroxylase (alkB). Microcosms bioaugmented with Enterobacter, Pandoraea and Burkholderia strains showed efficient biodegradation (98% TPH removal) well fitted in first order kinetic model with low rate constants and decreased half-life. This study proves that catabolically efficient bacteria resides naturally in complex petroleum refinery wastes and those can be useful for bioaugmentation based bioremediation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 242, October 2017, Pages 15-27
نویسندگان
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