Quantitative characterization of enzyme adsorption and hydrolytic performance for ultrafine grinding pretreated corn stover

- Ultrafine grinding is efficient in changing the microstructure properties.
- The quantitative analysis of enzyme adsorption and hydrolysis were presented.
- The enzyme adsorption was exponential to hydrolytic production for UGCS.
- The binding enzyme proportion was inversely proportional to enzyme consumption.

Quantitative analysis of enzyme adsorption and hydrolysis were performed for sieve-based grinding corn stover (SGCS) and ultrafine grinding corn stover (UGCS)1 with different enzyme consumptions. The UGCS presented significantly higher enzyme adsorption quantity (5.15 mg/g for UGCS, 1.33 mg/g for SGCS), higher glucose yield (49.75% for UGCS, 28.75% for SGCS) under 20 FPU/g and higher binding enzyme proportion (41.32% for UGCS, 10.64% for SGCS under 5 FPU/g) which can be attributed to the more accessible microstructure properties. The relationship between enzyme adsorption and hydrolytic production was directly proportional for SGCS (GY1 = 21.04 × AQ1 + 1.86 (R2 = 0.95)) while was exponential for UGCS (GY2 = 49.42 × (1 − e−0.57×AQ2) (R2 = 0.99)),2 indicating that overmuch enzyme consumption was not advisable for UGCS at economical aspect.

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