Simultaneous microbial reduction of vanadium (V) and chromium (VI) by Shewanella loihica PV-4

- Simultaneous reduction of V(V) and Cr(VI) by Shewanella loihica PV-4 is realized.
- Enhancement of Cr(VI) bioreduction is observed with the suppressed V(V) reduction.
- Both membrane components and cytoplasmic fractions contribute to these reductions.
- Most Cr(VI) is reduced extracellularly, V(V) tends to be reduced intracellularly.

Toxic vanadium (V) and chromium (VI) often co-exist in wastewater from vanadium ore smelting and their reductions by bacterial strain Shewanella loihica PV-4 is realized simultaneously. After 27-d operation, 71.3% of V(V) and 91.2% of Cr(VI) were removed respectively, with citrate as organic carbon source. Enhancement of Cr(VI) bioreduction was observed with the suppressed V(V) reduction. V(IV) and Cr(III), the main reduction products, precipitated inside the organisms and attached on cell surfaces. Both membrane components containing cytochrome c and cytoplasmic fractions containing soluble proteins as well as NADH may contribute to these microbial reductions. Most Cr(VI) were reduced extracellularly and V(V) tended to be reduced through intracellular process, as revealed by mapping the microbial surface and a line scan across the cell, performed by scanning transmission electron microscopy. This study provides an efficient alternative for controlling combined pollution caused by these two metals based on microbial technology.