کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5030774 | 1470932 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Competition-derived FRET-switching cationic conjugated polymer-Ir(III) complex probe for thrombin detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Competition-derived FRET-switching cationic conjugated polymer-Ir(III) complex probe for thrombin detection Competition-derived FRET-switching cationic conjugated polymer-Ir(III) complex probe for thrombin detection](/preview/png/5030774.png)
چکیده انگلیسی
A novel, label-free and convenient strategy for thrombin assay has been developed based on the fluorescence resonance energy transfer (FRET) from a cationic conjugated polymer (CCP) to Ir(III) complex. The energy donor (CCP) and acceptor (Ir(III) complex) were taken into close proximity through Ï-Ï stacking interaction and electrostatic interaction, leading to the occurrence of FRET. However, the introduction of the thrombin aptamer upset the status and blocked the FRET process, but afterwards the reappearance of FRET phenomenon was confirmed by the special binding interaction between aptamer and thrombin, thus achieving the quantitative detection of thrombin. This assay could detect thrombin as low concentration as about 0.05Â pM and provided a highly specific selectivity among other nonspecific proteins. Moreover, the strategy may allow our platform to provide similar sensitivity toward different targets in an aptamer-structure-independent manner. Furthermore, the assay can be used to detect thrombin in diluted real urine or serum samples with a satisfactory recovery, implying its great potential for rapid detection of thrombin in the clinic.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 100, 15 February 2018, Pages 132-138
Journal: Biosensors and Bioelectronics - Volume 100, 15 February 2018, Pages 132-138
نویسندگان
Chunnuan Du, Yufang Hu, Qingqing Zhang, Zhiyong Guo, Guoping Ge, Sui Wang, Chunyang Zhai, Mingshan Zhu,