کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5030893 1470934 2017 25 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biochemical-immunological hybrid biosensor based on two-dimensional chromatography for on-site sepsis diagnosis
ترجمه فارسی عنوان
بیوسنسور هیبرید بیوشیمیایی ایمونولوژیک بر اساس کروماتوگرافی دو بعدی برای تشخیص سپسیس در محل
کلمات کلیدی
آنتی بیوتیک ها، بیوسنزینگ چندگانه کروماتوگرافی دو بعدی، آشکارساز تلفن هوشمند، تشخیص سریع سپسیس،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A hybrid-biosensor system that can simultaneously fulfill the immunoassay for protein markers (e.g., C-reactive protein (CRP) and procalcitonin (PCT)) and the enzyme assay for metabolic substances (e.g., lactate) in the same sepsis-based sample has been devised. Such a challenge was pursued through the installation of an enzyme-reaction zone on the signal pad of the typical immuno-strip for the rapid two-dimensional (2-D)-chromatography test. To minimize the mutual interference in the hybrid assays, a pre-determined membrane site was etched in a pattern and mounted with a biochemical-reaction pad, thereby allowing a loaded sample to enter and then stay in the pad for a colored-signal production over the course of an immunoassay. By employing such a constructed system, a serum sample was analyzed according to the vertical direction flowing along the strip, which supplied lactate to the biochemical-reaction zone and then protein markers to the immunological-binding area that was pre-coated with capture antibodies. Thereafter, the enzyme-signal tracers for the immunoassay and the substrate solution were sequentially furnished using a horizontal path for the tracing of the immune complexes that were formed with CRP or PCT. The color signal that was produced from each assay was detected at a pre-determined time and quantified on a smartphone-based detector. Under the optimal conditions, the dynamic ranges for the analytes covered the respective clinical ranges, and the total coefficient of variation was between 8.6% and 13.3%. The hybrid biosensor further showed a high correlation (R2 > 0.95) with the reference systems for the target markers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 98, 15 December 2017, Pages 7-14
نویسندگان
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