کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5030934 1470934 2017 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical DNA biosensor based on grafting-to mode of terminal deoxynucleoside transferase-mediated extension
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical DNA biosensor based on grafting-to mode of terminal deoxynucleoside transferase-mediated extension
چکیده انگلیسی
Previously reported electrochemical DNA biosensors based on in-situ polymerization approach reveal that terminal deoxynucleoside transferase (TdTase) has good amplifying performance and promising application in the design of electrochemical DNA biosensor. However, this method, in which the background is significantly affected by the amount of TdTase, suffers from being easy to produce false positive result and poor stability. Herein, we firstly present a novel electrochemical DNA biosensor based on grafting-to mode of TdTase-mediated extension, in which DNA targets are polymerized in homogeneous solution and then hybridized with DNA probes on BSA-based DNA carrier platform. It is surprising to find that the background in the grafting-to mode of TdTase-based electrochemical DNA biosensor have little interference from the employed TdTase. Most importantly, the proposed electrochemical DNA biosensor shows greatly improved detection performance over the in-situ polymerization approach-based electrochemical DNA biosensor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 98, 15 December 2017, Pages 345-349
نویسندگان
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