کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5030951 | 1470934 | 2017 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bridged Rebar Graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We report a novel fabrication method of functionalised Bridged Rebar Graphene (BRG) onto newly designed nanostructured aptasensor for label free impedimetric sensing of pathogenic bacteria E. coli O78:K80:H11. The chemical facilitated unscrolling of MWCNT and subsequent bridging with terephthalaldehyde (TPA) to form 3D-hierarchical BRG nanoconstruct exhibited synergistic effect by combining enhanced electrical properties and facile chemical functionality for stable bio-interface. The bacteria-DNA interactions were captured on BRG nanostructured electrode by using specific anti-E.coli DNA aptamer (Kd~ 14Â nM), screened by new in-situ developed SELEX method using phenylboronic acid on microtitre plate. The developed nanostructured aptasensor demonstrated a low detection limit and sensitivity of ~ 101Â cfu/mL towards E. coli O78:K80:H11 with a dynamic response range from 101 to 106Â cfu/mL in water, juice and milk samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 98, 15 December 2017, Pages 486-493
Journal: Biosensors and Bioelectronics - Volume 98, 15 December 2017, Pages 486-493
نویسندگان
Harmanjit Kaur, Munish Shorie, Manju Sharma, Ashok K. Ganguli, Priyanka Sabherwal,