کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5030978 | 1470935 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrodepositable alginate membranes for enzymatic sensors: An amperometric glucose biosensor for whole blood analysis
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Electrodepositable alginate membranes for enzymatic sensors: An amperometric glucose biosensor for whole blood analysis Electrodepositable alginate membranes for enzymatic sensors: An amperometric glucose biosensor for whole blood analysis](/preview/png/5030978.png)
چکیده انگلیسی
Simple and disposable point of care systems are usually the best solution for chronic patients to get a rapid diagnosis in home care context. However, their main drawback relies on the poor reliability derived from the low stability of the bio-recognition elements and low quality of the transducers. In the current work, we study the use of electrodeposited calcium alginate hydrogels as a biocompatible matrix in the development of enzymatic amperometric biosensors for whole blood analysis, to enhance the enzymes stability and to protect the transducer from biofouling. The alginate electrodeposition involves the controlled Ca2+ release, so the gel thickness can be modulated. In the biosensor, horseradish peroxidase (HRP) and glucose oxidase (GOD) were electrodeposited within the hydrogel and the activity of the bi-enzymatic system was analyzed chronoamperometrically using 3,3â²,5,5â²-Tetramethylbenzidine (TMB) as the mediator. Besides enzyme entrapment, the obtained gels protected the transducer from biofouling, enabling the reuse of the transducer after hydrogel removal and re-electrodeposition. The biosensors showed good analytical characteristics to glucose determination in whole blood samples, discriminating among healthy and hyperglycemic samples, with good sensitivity (â 0.27 µA cmâ2 mMâ1), low limit of detection (126 µM) and long lineal range (2-12 mM).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 97, 15 November 2017, Pages 136-142
Journal: Biosensors and Bioelectronics - Volume 97, 15 November 2017, Pages 136-142
نویسندگان
A. Márquez, C. Jiménez-Jorquera, C. DomÃnguez, X. Muñoz-Berbel,