کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5030997 | 1470935 | 2017 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Colorimetric sensor array for protein discrimination based on different DNA chain length-dependent gold nanoparticles aggregation
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A facile colorimetric sensor array for detection of proteins was demonstrated using DNA as nonspecific receptors. We found that different proteins could trigger the DNA-protected gold nanoparticles (AuNPs) to show different aggregation behaviors in the presence of salt with high concentrations along with various color changes. As a proof-of-concept application, a multi-protein discrimination array was fabricated with two ssDNA strands (15A and 30A bases) as a receptor array. The combinatorial colorimetric response of this sensor array can be analyzed by linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). 12 proteins have been well distinguished with the naked eye at the 50Â nM level. The proteins in human serum have also been discriminated. Furthermore, the accuracies of discrimination of the similar mixtures of proteins and unknown samples were all 100%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 97, 15 November 2017, Pages 332-337
Journal: Biosensors and Bioelectronics - Volume 97, 15 November 2017, Pages 332-337
نویسندگان
Xiangcong Wei, Yuxian Wang, Yingxin Zhao, Zhengbo Chen,