کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031037 1470938 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dual-responsive electrochemical immunosensor for prostate specific antigen detection based on Au-CoS/graphene and CeO2/ionic liquids doped with carboxymethyl chitosan complex
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Dual-responsive electrochemical immunosensor for prostate specific antigen detection based on Au-CoS/graphene and CeO2/ionic liquids doped with carboxymethyl chitosan complex
چکیده انگلیسی
Combining dual-signal channels of differential pulse voltammetry (DPV) and amperometric i-t curve, we developed an amplified sandwich-type electrochemical immunosensor for ultrasensitive detection of prostate specific antigen (PSA). Due to the large specific surface area and good adsorption property, 3-aminopropyltriethox functionalized CeO2 mesoporous nanoparticles (NH2-M-CeO2) was used for supporting toluidine blue (TB) as the electron transfer mediator and anti-PSA as the signal response. To prevent the leak of TB and facilitate the electron transfer property of NH2-M-CeO2, ionic liquids doped carboxymethyl chitosan (CMC/ILs) were incorporated and TB/M-CeO2/CMC/ILs complex was gotten to act as the label of anti-PSA (Ab2). Meanwhile, Au-CoS/graphene was as the matrix materials modifying the electrode to immobilize anti-PSA (Ab1). Specifically, Au-CoS/graphene cannot produce electrochemical signals through DPV method but can provide an obviously electrochemical signal towards catalysis hydrogen peroxide (H2O2) through amperometric i-t curve method. At the time of detection, with the increase of concentrations of PSA, the DPV signals from TB/M-CeO2/CMC/ILs were increased and the amperometric i-t curve signals from the Au-CoS/graphene were decreased. Under the optimum conditions, the immunosensor demonstrated remarkable analytical performance of a linear range of 0.5 pg/mL to 50 ng/mL with a detection limit of 0.16 pg/mL for quantitative detection of PSA (S/N=3).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 94, 15 August 2017, Pages 141-147
نویسندگان
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