کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031046 1470938 2017 30 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanosphere-based one-step strategy for efficient and nondestructive detection of circulating tumor cells
ترجمه فارسی عنوان
استراتژی یک مرحله ای مبتنی بر نانوسفر برای تشخیص کارآمد و غیرتشخیص سلول های توموری در گردش است
کلمات کلیدی
نانوسر مغناطیسی، نانوسفر فلورسنت، تشخیص یک مرحله ای، کارآمد، دوستانه همراه، سلول های تومور در معرض عمر مفید،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Detecting viable circulating tumor cells (CTCs) without disruption to their functions for in vitro culture and functional study could unravel the biology of metastasis and promote the development of personalized anti-tumor therapies. However, existing CTC detection approaches commonly include CTC isolation and subsequent destructive identification, which damages CTC viability and functions and generates substantial CTC loss. To address the challenge of efficiently detecting viable CTCs for functional study, we develop a nanosphere-based cell-friendly one-step strategy. Immunonanospheres with prominent magnetic/fluorescence properties and extraordinary stability in complex matrices enable simultaneous efficient magnetic capture and specific fluorescence labeling of tumor cells directly in whole blood. The collected cells with fluorescent tags can be reliably identified, free of the tedious and destructive manipulations from conventional CTC identification. Hence, as few as 5 tumor cells in ca. 1 mL of whole blood can be efficiently detected via only 20 min incubation, and this strategy also shows good reproducibility with the relative standard deviation (RSD) of 8.7%. Moreover, due to the time-saving and gentle processing and the minimum disruption of immunonanospheres to cells, 93.8±0.1% of detected tumor cells retain cell viability and proliferation ability with negligible changes of cell functions, capacitating functional study on cell migration, invasion and glucose uptake. Additionally, this strategy exhibits successful CTC detection in 10/10 peripheral blood samples of cancer patients. Therefore, this nanosphere-based cell-friendly one-step strategy enables viable CTC detection and further functional analyses, which will help to unravel tumor metastasis and guide treatment selection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 94, 15 August 2017, Pages 219-226
نویسندگان
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