کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031202 1470941 2017 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Design an aptasensor based on structure-switching aptamer on dendritic gold nanostructures/Fe3O4@SiO2/DABCO modified screen printed electrode for highly selective detection of epirubicin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Design an aptasensor based on structure-switching aptamer on dendritic gold nanostructures/Fe3O4@SiO2/DABCO modified screen printed electrode for highly selective detection of epirubicin
چکیده انگلیسی
The present work describes a label free electrochemical aptasensor for selective detection of epirubicin. In this project, 5′-thiole terminated aptamer was self-assembled on carbon screen printed electrode, which modified with electrodeposited gold nanoparticles on magnetic double-charged diazoniabicyclo [2.2.2] octane dichloride silica hybrid (Fe3O4@SiO2/DABCO) by Au-S bond. The interactions of epirubicin with aptamer on the AuNPs/Fe3O4@SiO2/DABCO/SPE have been studied by cyclic voltammetry, linear sweep voltammetry and electrochemical impedance spectroscopy. Under optimized conditions, the peak current of epirubicin increased linearly with increasing epirubicin concentration, due to the switching in the aptamer conformation and formation of aptamer- epirubicin complex instead of aptamer on the modified electrode surface. The Apt/AuNPs/Fe3O4@SiO2/DABCO/SPE is sensitive, selective and has two linear range from 0.07µM to 1.0 µM and 1.0 µM to 21.0µM with a detection limit of 0.04 µM. The applicability of the aptasensor was successfully assessed by determination of epirubicin in a human blood serum sample.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 91, 15 May 2017, Pages 650-657
نویسندگان
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