کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031224 | 1470942 | 2017 | 52 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and forensic identification. In the past three decades, PCR has evolved from end-point PCR, through real-time PCR, to its current version, which is the absolute quantitive digital PCR (dPCR). In this review, we first discuss the principles of all key steps of dPCR, i.e., sample dispersion, amplification, and quantification, covering commercialized apparatuses and other devices still under lab development. We highlight the advantages and disadvantages of different technologies based on these steps, and discuss the emerging biomedical applications of dPCR. Finally, we provide a glimpse of the existing challenges and future perspectives for dPCR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 459-474
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 459-474
نویسندگان
Lei Cao, Xingye Cui, Jie Hu, Zedong Li, Jane Ru Choi, Qingzhen Yang, Min Lin, Ying Hui Li, Feng Xu,