کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031244 1470942 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multifunctional nanoparticles for protein detections in thin channels
ترجمه فارسی عنوان
نانوذرات چند منظوره برای تشخیص پروتئین در کانال های نازک
کلمات کلیدی
ایمن مغناطیسی، نانوذرات بیوفیزیکی، کانال نازک
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This paper presents a method for simultaneous detection of two proteins by using multifunctional nanoparticles with a magnetic immunoassay in thin channels. Biofunctional magnetic graphene quantum dots (GQDs) combined with two biofunctional quantum dots (QDs) were used for simultaneously detecting two proteins. Magnetic GQDs enabled selective and quantitative nanoparticle deposition with blue emission. Biofunctional QDs confirmed the two protein detections with orange and green emissions. We used two model biomarkers [alpha-fetoprotein (AFP) and cancer antigen 125 (CA125)] to demonstrate the feasibility of the proposed method. The detection limits (0.06 pg/mL AFP and 0.001 U/mL CA125) and linear ranges (0.2 pg/mL-0.68 ng/mL AFP and 0.003-25 U/mL CA125) of this method are the same as those of single protein detection within experimental errors. These detection limits are substantially lower and the linear ranges are considerably wider than those of enzyme-linked immunosorbent assay (ELISA) and other immunoassay methods. The differences between the proposed method and an ELISA method in AFP and CA125 measurements of serum samples were less than 12%. The proposed method demonstrates favorable detection of biomarkers with advantages of speed, sensitivity, selectivity, and throughput.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 153-158
نویسندگان
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