کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031280 1470942 2017 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A fluorescent carbon-dots-based mitochondria-targetable nanoprobe for peroxynitrite sensing in living cells
ترجمه فارسی عنوان
یک نانوپرداز قابل تنظیم برای میتوکندری های فلورسنت کربن برای اندازه گیری پراکسینتریت در سلول های زنده
کلمات کلیدی
نقاط کربن، هدفمند سازی میتوکندریا، تصویربرداری فلورسنت، نانوکامپوزیت، پروکسینیتریت،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Mitochondria, the power generators in cell, are a primary organelle of oxygen consumption and a main source of reactive oxygen/nitrogen species (ROS/RNS). Peroxynitrite (ONOO−), known as a kind of RNS, has been considered to be a significant factor in many cell-related biological processes, and there is great desire to develop fluorescent probes that can sensitively and selectively detect peroxynitrite in living cells. Herein, we developed a fluorescent carbon-dots (C-dots) based mitochondria-targetable nanoprobe with high sensitivity and selectivity for peroxynitrite sensing in living cells. The C-dots with its surface rich in amino groups was synthesized using o-phenylenediamine as carbon precursor, and it could be covalently conjugated with a mitochondria-targeting moiety, i.e. triphenylphosphonium (TPP). In the presence of peroxynitrite, the fluorescence of the constructed nanoprobe (C-dots-TPP) was efficiently quenched via a mechanism of photoinduced electron transfer (PET). The nanoprobe exhibited relatively high sensitivity (limit of detection: 13.5 nM) and selectivity towards peroxynitrite in aqueous buffer. The performance of the nanoprobe for fluorescence imaging of peroxynitrite in mitochondria was investigated. The results demonstrated that the nanoprobe showed fine mitochondria-targeting ability and imaging contrast towards peroxynitrite in living cells. We anticipate that the proposed nanoprobe will provide a facile tool to explore the role played by peroxynitrite in cytobiology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 501-507
نویسندگان
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