کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031324 | 1470933 | 2018 | 7 صفحه PDF | دانلود رایگان |
- An enzymatic keypad lock with a bipolar electrode was developed for the first time.
- The biomolecular keypad lock system could be easily reset.
- The keypad lock could be reprogrammed when the original password was destroyed.
- The keypad lock demonstrated good practicability with its visible output signals.
Herein, a resettable and reprogrammable biomolecular keypad lock on the basis of a closed bipolar electrode (BPE) system was established. In this system, one ITO electrode with immobilized chitosan (CS) and glucose oxidase (GOD), designated as CS-GOD, acted as one pole of BPE in the sensing cell; another ITO with electrodeposited Prussian blue (PB) films as the other pole in the reporting cell. The addition of ascorbic acid (AA) in the sensing cell with driving voltage (Vtot) at +2.5Â V would make the PB films become Prussian white (PW) in the reporting cell, accompanied by the color change from blue to nearly transparent. On the other hand, with the help of oxygen, the addition of glucose in the sensing cell with Vtot at â1.5Â V would induce PW back to PB. The change of color and the corresponding UV-vis absorbance at 700Â nm for the PB/PW films in the reporting cell could be reversibly switched by changing the solute in the sensing cell between AA and glucose and then switching Vtot between +2.5 and â1.5Â V. Based on these, a keypad lock was developed with AA, glucose and Vtot as 3 inputs, and the color change of the PB/PW films as the output. This keypad lock system combined enzymatic catalysis with bipolar electrochemistry, demonstrating some unique advantages such as good reprogrammability, easy resettability and visual readout by naked eye.
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 163-169