کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5031372 | 1470933 | 2018 | 7 صفحه PDF | دانلود رایگان |
- By a simple electrostatic self-assembly method, É-polylysine-heparin microparticles which possess uniform particle size, good dispersibility and moderate long-term anticoagulation capability have been compounded.
- The É-polylysine-heparin microparticles were used to fabricate a Lipase-(É-polylysine-heparin)-GCE electrode and proposed Lipase-(É-polylysine-heparin)-GCE electrode had strong anti-interference ability and a long shelf-life.
- Combining moderate long-term anticoagulation capability and microsized effect, the proposed biosensor had good antithrombosis ability and could be directly used in whole blood, which provides new analytical systems for clinical illness diagnosis.
Triglycerides are clinically important marker for atherosclerosis, heart disease and hypertension. Here, a platform for detecting triglycerides in whole blood directly was developed based on hemocompatible É-polylysine-heparin microparticles. The obtained products of É-polylysine-heparin microparticles were characterized by fourier transform infrared (FT-IR) spectra, transmission electron microscopy (TEM) and ζ-potential. Moreover, the blood compatibility of É-polylysine-heparin microparticles was characterized by in vitro coagulation tests, hemolysis assay and whole blood adhesion tests. Considering of uniform particle size, good dispersibility and moderate long-term anticoagulation capability of the microparticles, a Lipase-(É-polylysine-heparin)-glassy carbon electrode (GCE) was constructed to detect triglycerides. The proposed biosensor had good electrocatalytic activity towards triglycerides, in which case the sensitivity was 0.40 μA mgâ1 dL cmâ2 and the detection limit was 4.67 mg dLâ1 (S/N = 3). Meanwhile, the Lipase-(É-polylysine-heparin)-GCE electrode had strong anti-interference ability as well as a long shelf-life. Moreover, for the detection of triglycerides in whole blood directly, the detection limit was as low as 5.18 mg dLâ1. The new constructed platform is suitable for detecting triglycerides in whole blood directly, which provides new analytical systems for clinical illness diagnosis.
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 571-577