کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031422 | 1470943 | 2017 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
ترجمه فارسی عنوان
مقایسه جریان جانبی اسید نوکلئیک و ژنوزانسینگ الکتروشیمیایی برای تشخیص همزمان پاتوژن های خوراکی
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کلمات کلیدی
جریان جانبی جانبی اسید نوکلئیک، الکتروشیمیایی مگنتو ژنوسانسینگ، باکتری مواد غذایی، تشخیص همزمان، ذرات مغناطیسی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Due to the increasing need of rapid tests for application in low resource settings, WHO summarized their ideal features under the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, Delivered to those who need it). In this work, two different platforms for the rapid and simultaneous testing of the foodborne pathogens E. coli O157:H7 and Salmonella enterica, in detail a nucleic acid lateral flow and an electrochemical magneto-genosensor are presented and compared in terms of their analytical performance. The DNA of the bacteria was amplified by polymerase chain reaction using a quadruple-tagging set of primers specific for E. coli eaeA (151Â bp) and Salmonella enterica yfiR (375Â bp) genes. During the amplification, the amplicons were labelled at the same time with biotin/digoxigenin or biotin/fluorescein tags, respectively. The nucleic acid lateral flow assay was based on the use of streptavidin gold nanoparticles for the labelling of the tagged amplicon from E. coli and Salmonella. The visual readout was achieved when the gold-modified amplicons were captured by the specific antibodies. The features of this approach are discussed and compared with an electrochemical magneto-genosensor. Although nucleic acid lateral flow showed higher limit of detection, this strategy was able to clearly distinguish positive and negative samples of both bacteria being considered as a rapid and promising detection tool for bacteria screening.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 88, 15 February 2017, Pages 265-272
Journal: Biosensors and Bioelectronics - Volume 88, 15 February 2017, Pages 265-272
نویسندگان
A. Ben Aissa, J.J. Jara, R.M. Sebastián, A. Vallribera, S. Campoy, M.I. Pividori,