کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031518 | 1470939 | 2017 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A new lateral flow strip assay (LFSA) using a pair of aptamers for the detection of Vaspin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A new lateral flow strip assay (LFSA) using a pair of aptamers for the detection of Vaspin A new lateral flow strip assay (LFSA) using a pair of aptamers for the detection of Vaspin](/preview/png/5031518.png)
چکیده انگلیسی
A new lateral flow strip assay (LFSA) using a pair of aptamers has been designed and successfully developed using a pair of aptamers-functionalized with gold nanoparticles (AuNPs). This new LFSA biosensor system utilizes a cognate aptamer duo binding to vaspin, a target protein, at the two different binding sites, and exhibited a sensitive and highly selective response to vaspin. A sandwich-type format in LFSA was developed based on biotin-labeled primary V1 aptamer immobilized on streptavidin coated membrane as a capturing probe and secondary V49 aptamer conjugated with AuNPs as a signaling probe. Using this LFSA, vaspin could be visibly observed within the detectable concentrations of vaspin up to 5Â nM in both buffer and serum conditions. The sensitivity of this LFSA developed in this study was ranged from 0.137 to 25Â nM in buffer and from 0.105 to 25Â nM in spiked human serum, respectively. The limit of detection (LOD) of this LFSA was found to be 0.137Â nM and 0.105Â nM in buffer and spiked human serum condition, respectively. Therefore, this study has shown successful development of a simple yet effective LFSA for vaspin detection, and this development is not only limited to this target, but also other targets with a pair of aptamers available, without any special and laborious instrumentations. This system will be particularly useful as a screening tool for rapid on-site detection of any targets with a pair of aptamers generated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 93, 15 July 2017, Pages 21-25
Journal: Biosensors and Bioelectronics - Volume 93, 15 July 2017, Pages 21-25
نویسندگان
Nurul Hanun Ahmad Raston, Van-Thuan Nguyen, Man Bock Gu,