کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031640 1470940 2017 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Self-assembled polymeric nanoparticles film stabilizing gold nanoparticles as a versatile platform for ultrasensitive detection of carcino-embryonic antigen
ترجمه فارسی عنوان
فیلم نانوذرات پلیمری خودآموزی، پایدار ساختن نانوذرات طلا به عنوان یک پلت فرم چند منظوره برای تشخیص حساسیت بالا از آنتیژن کارسینوژنز جنینی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this work, a novel impedimetric immunosensor was developed based on electrophoretic deposition of polymeric self-assembled nanoparticles for the sensitive determination of carcino-embryonic antigen (CEA). Biocompatible polymeric nanoparticles γ-PGA-DA@CS were prepared by self-assembly of chitosan (CS) and dopamine modified poly(γ-glutamic acid) (γ-PGA-DA) under mild conditions. A dense and nanostructured nanoparticles film was obtained on the electrode surface by electrophoretic deposition of γ-PGA-DA@CS nanoparticles. Gold nanoparticles (Au NPs) were then tightly anchored on γ-PGA-DA@CS film with homogeneous dispersion due to numerous exposed dopamine adhesive dots present on the surface of γ-PGA-DA@CS. The obtained Au/γ-PGA-DA@CS nanocomposite film not only increases the electrode surface area in nanoscale dimension, but also provides a highly stable and biocompatible matrix for the convenient conjugation of antibody, thus providing a high-efficiency immunoassay platform. Monoclonal antibodies to carcinoembryonic antigen (CEA-Ab) were effectively immobilized on the Au/γ-PGA-DA@CS film and a label-free impedimetric immunosensor was fabricated successfully as the ultimate goal. Under optimal conditions, the resultant immunosensor exhibited a wide linear range from 2.0×10-14 g mL−1 to 2.0×10−8 g mL−1 for the detection of CEA with a low detection limit of 10 fg mL−1. To the best of our knowledge, this was the lowest detection limit compared with other counterparts of label-free impedimetric immunosensors. Moreover, the immunosensor showed high specificity, good stability and satisfactory reproducibility. As a proof of concept, the proposed strategy provided a promising and versatile platform for clinical immunoassay of other tumor markers and biomolecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 92, 15 June 2017, Pages 570-576
نویسندگان
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