کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031640 | 1470940 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Self-assembled polymeric nanoparticles film stabilizing gold nanoparticles as a versatile platform for ultrasensitive detection of carcino-embryonic antigen
ترجمه فارسی عنوان
فیلم نانوذرات پلیمری خودآموزی، پایدار ساختن نانوذرات طلا به عنوان یک پلت فرم چند منظوره برای تشخیص حساسیت بالا از آنتیژن کارسینوژنز جنینی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this work, a novel impedimetric immunosensor was developed based on electrophoretic deposition of polymeric self-assembled nanoparticles for the sensitive determination of carcino-embryonic antigen (CEA). Biocompatible polymeric nanoparticles γ-PGA-DA@CS were prepared by self-assembly of chitosan (CS) and dopamine modified poly(γ-glutamic acid) (γ-PGA-DA) under mild conditions. A dense and nanostructured nanoparticles film was obtained on the electrode surface by electrophoretic deposition of γ-PGA-DA@CS nanoparticles. Gold nanoparticles (Au NPs) were then tightly anchored on γ-PGA-DA@CS film with homogeneous dispersion due to numerous exposed dopamine adhesive dots present on the surface of γ-PGA-DA@CS. The obtained Au/γ-PGA-DA@CS nanocomposite film not only increases the electrode surface area in nanoscale dimension, but also provides a highly stable and biocompatible matrix for the convenient conjugation of antibody, thus providing a high-efficiency immunoassay platform. Monoclonal antibodies to carcinoembryonic antigen (CEA-Ab) were effectively immobilized on the Au/γ-PGA-DA@CS film and a label-free impedimetric immunosensor was fabricated successfully as the ultimate goal. Under optimal conditions, the resultant immunosensor exhibited a wide linear range from 2.0Ã10-14 g mLâ1 to 2.0Ã10â8 g mLâ1 for the detection of CEA with a low detection limit of 10 fg mLâ1. To the best of our knowledge, this was the lowest detection limit compared with other counterparts of label-free impedimetric immunosensors. Moreover, the immunosensor showed high specificity, good stability and satisfactory reproducibility. As a proof of concept, the proposed strategy provided a promising and versatile platform for clinical immunoassay of other tumor markers and biomolecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 92, 15 June 2017, Pages 570-576
Journal: Biosensors and Bioelectronics - Volume 92, 15 June 2017, Pages 570-576
نویسندگان
Sheng Xu, Rongli Zhang, Wei Zhao, Ye Zhu, Wei Wei, Xiaoya Liu, Jing Luo,