کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031737 | 1369954 | 2017 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fabrication of peptide stabilized fluorescent gold nanocluster/graphene oxide nanocomplex and its application in turn-on detection of metalloproteinase-9
ترجمه فارسی عنوان
ساخت نانوکامپپلکس نانوکیلستر طلائی / گرافن اکسید فلورسنت تثبیت کننده پپتید و کاربرد آن در تشخیص روشن شدن فلزوپروتئیناز-9
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کلمات کلیدی
نانوکیلر طلا، اکسید گرافن، پپتیده متالوپروتئیناز-9، متاستاز سرطان، سایت فکری خنک کننده فلورسنت،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This study introduces the double-ligands stabilizing gold nanoclusters and the fabrication of gold nanocluster/graphene nanocomplex as a “turn-on” fluorescent probe for the detection of cancer-related enzyme matrix metalloproteinase-9. A facile, one-step approach was developed for the synthesis of fluorescent gold nanoclusters using peptides and mercaptoundecanoic acid as co-templating ligands. The peptide was designed to possess a metalloproteinase-9 cleavage site and to act not only as a stabilizer but also as a targeting ligand for the enzyme detection. The prepared gold nanoclusters show an intense red fluorescence with a broad adsorption spectrum. In the presence of the enzyme, due to the excellent quenching properties and the negligible background of graphene oxide, the developed peptide-gold nanocluster/graphene nanocomplex yielded an intense “turn-on” fluorescent response, which strongly correlated with the enzyme concentration. The limit of detection of the nanocomplex was 0.15Â nM. The sensor was successfully applied for “turn-on” detection of metalloproteinase-9 secreted from human breast adenocarcinoma MCF-7 cells with high sensitivity, selectivity, significant improvement in terms of detection time and simplicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 89, Part 1, 15 March 2017, Pages 666-672
Journal: Biosensors and Bioelectronics - Volume 89, Part 1, 15 March 2017, Pages 666-672
نویسندگان
Phuong-Diem Nguyen, Vu Thanh Cong, Changyoon Baek, Junhong Min,