High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles

- Innovative biocatalyst with good properties to produce invert sugar.
- mDE-APTES-invertase showed 92.5% of residual specific activity.
- High sucrolytic activity (3358 U mg−1 protein) by mDE-APTES-invertase was obtained.
- Remarkable results of thermal and storage stability, and reuse for mDE-APTES-invertase were found.

Invertase immobilized on magnetic diatomaceous earth nanoparticles (mDE-APTES-invertase) with high sucrolytic activity was obtained by an easy and low-cost method. An experimental design was carried out to investigate the best immobilization conditions and it allowed obtaining an immobilized derivative with a residual specific activity equal to 92.5%. Then, a second experimental design selected the mDE-APTES-invertase with higher specific activity in relation to other derivatives reported in the literature (2.42-fold). Thermal and storage stability for immobilized invertase were found to be 35 °C for 60 min (85% retained activity) and 120 days storage period (80% retained activity), respectively. Besides, a residual activity higher than 60% and 50% were observed for mDE-APTES-invertase after reuse in short and long term, respectively. Given the simple and efficient method to obtain an immobilized derivative with high activity, the mDE nanoparticles appear to be a promising matrix for invertase immobilization as well as for other biomolecules.

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