Ethanol-dispersed and antibody-conjugated polymer nanofibers for the selective capture and 3-dimensional culture of EpCAM-positive cells

Polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) electrospun nanofibers (NFs) conjugated by anti-EpCAM monoclonal antibody is presented as an integrative platform for target cell capture and culture. The developed NFs has inter-fiber space for efficient selective cell capturing in wide concentration range of cell containing suspensions. Captured cells could be directly cultured in the NFs matrix, to ensure a cell culture environment.91