AntihypoxamiR functionalized gramicidin lipid nanoparticles rescue against ischemic memory improving cutaneous wound healing

Successful intervention of non-healing ischemic wound is a major problem. Presence of hypoxia induced miR-210 limits keratinocytes proliferation and compromised wound closure. In this work, we report the formulation of antihypoxamiR functionalized gramicidin lipid nanoparticles (AFGLN). A single intradermal delivery of AFGLN encapsulating LNA-conjugated anti-hypoximiR-210 (AFGLNmiR-210) lowered miR-210 level in the ischemic wound-edge tissue. AFGLNmiR-210 delivery elevated ATP content at the ischemic wound-edge tissue recovering bioenergetics necessary for keratinocyte proliferation. Consistently, AFGLNmiR-210 improved ischemic wound closure. The nanoparticle based approach reported herein is effective for miR-directed wound therapeutics warranting further translational development.247