کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5131783 | 1491332 | 2016 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isothermal titration calorimetry for drug design: Precision of the enthalpy and binding constant measurements and comparison of the instruments
ترجمه فارسی عنوان
کالری سنجی تیتانیت ایزوترمال برای طراحی دارو: دقت اندازه گیری های آنتالپی و اتصال ثابت و مقایسه ابزار
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Isothermal titration calorimetry (ITC) is one of the most robust label- and immobilization-free techniques used to measure protein - small molecule interactions in drug design for the simultaneous determination of the binding affinity (ÎG) and the enthalpy (ÎH), both of which are important parameters for structure-thermodynamics correlations. It is important to evaluate the precision of the method and of various ITC instrument models by performing a single well-characterized reaction. The binding between carbonic anhydrase II and acetazolamide was measured by four ITC instruments - PEAQ-ITC, iTC200, VP-ITC, and MCS-ITC and the standard deviation of ÎG and ÎH was determined. Furthermore, the limit of an approach to reduce the protein concentration was studied for a high-affinity reaction (Kd = 0.3 nM), too tight to be measured by direct (non-displacement) ITC. Chemical validation of the enthalpy measurements is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 515, 15 December 2016, Pages 61-64
Journal: Analytical Biochemistry - Volume 515, 15 December 2016, Pages 61-64
نویسندگان
Vaida LinkuvienÄ, Georg Krainer, Wen-Yih Chen, Daumantas Matulis,