کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5131929 1378783 2017 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of low molecular weight compounds by MALDI imaging mass spectrometry - A tutorial review
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantification of low molecular weight compounds by MALDI imaging mass spectrometry - A tutorial review
چکیده انگلیسی


- This tutorial review focuses on the quantitative determination of compounds on tissue surfaces by MALDI-MS imaging.
- The article discusses and compares different normalization and calibration routines for quantification of small molecules.
- The review presents future MALDI-MSI developments regarding method harmonization, validation and reference materials.

Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) permits label-free in situ analysis of chemical compounds directly from the surface of two-dimensional biological tissue slices. It links qualitative molecular information of compounds to their spatial coordinates and distribution within the investigated tissue. MALDI-MSI can also provide the quantitative amounts of target compounds in the tissue, if proper calibration techniques are performed. Obviously, as the target molecules are embedded within the biological tissue environment and analysis must be performed at their precise locations, there is no possibility for extensive sample clean-up routines or chromatographic separations as usually performed with homogenized biological materials; ion suppression phenomena therefore become a critical side effect of MALDI-MSI. Absolute quantification by MALDI-MSI should provide an accurate value of the concentration/amount of the compound of interest in relatively small, well-defined region of interest of the examined tissue, ideally in a single pixel. This goal is extremely challenging and will not only depend on the technical possibilities and limitations of the MSI instrument hardware, but equally on the chosen calibration/standardization strategy. These strategies are the main focus of this article and are discussed and contrasted in detail in this tutorial review. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1865, Issue 7, July 2017, Pages 726-739
نویسندگان
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