The enrichment and characterization of ginger-derived glycoprotein using magnetic particles

- The lectin microarray was used to select lectins.
- Lectins selected were immobilized on magnetic particles.
- Five lectins (e.g. VVA, ConA, STL, LEL and LCA) were positive by lectin microarray.
- The structure of carbohydrate chains might contain GlaNAc, mannose, GlcNAc and LacNAc.

Ginger-derived glycoproteins are a widely distributed group of biological macromolecules with multiple functions. To date, the structure of ginger-derived glycoproteins has not been clarified with regard to their complexity, their sequence diversity and their uneven micro-distribution. In this study, a lectin microarray was used to evaluate 37 types of lectins and determine the optimal lectins that can conjugate with glycoproteins based on the fluorescence intensity. Subsequently, the lectins were immobilized on magnetic beads, coupled with glycoproteins to enrich ginger-derived glycoproteins, and evaluated using SDS-PAGE. Our results showed that five lectins (e.g. VVA, ConA, STL, LEL, and LCA) were selected by the lectin microarray and that VVA showed the highest fluorescence intensity. In addition, it is indicated that the structure of the carbohydrate chains might contain GlaNAc, mannose, GlcNAc, and LacNAc.