کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5133165 | 1492058 | 2017 | 7 صفحه PDF | دانلود رایگان |
- A natural E. coli O157:H7 specific bacteriophage was isolated and purified.
- No interference from S. aureus was observed.
- The method was applied to detect E. coli O157:H7 in spiked skim milk samples.
- It was the first time to utilize a natural phage for E. coli O157:H7 detection.
Mammal IgG antibodies are normally used in conventional immunoassays for E. coli O157:H7, which could lead to false positive results from the presence of protein A producing S. aureus. In this study, a natural specific bacteriophage was isolated and then conjugated with magnetic beads as a capture element in a sandwich format for the rapid and selective detection of E. coli O157:H7. To the best of our knowledge, it was the first time to utilize a natural bacteriophage to develop a phagomagnetic separation combined with colorimetric assay for E. coli O157:H7. The method has an overall time less than 2Â h with a detection limit of 4.9Â ÃÂ 104Â CFU/mL. No interference from S. aureus was observed. Furthermore, the proposed method was successfully applied to detect E. coli O157:H7 in spiked skim milk. The proposed detection system provided a potential method for E. coli O157:H7 and other pathogenic bacteria in food samples.
Journal: Food Chemistry - Volume 234, 1 November 2017, Pages 332-338