کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5133748 | 1492071 | 2017 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of calcium-binding peptide from Pacific cod (Gadus macrocephalus) bone on calcium bioavailability in rats Effect of calcium-binding peptide from Pacific cod (Gadus macrocephalus) bone on calcium bioavailability in rats](/preview/png/5133748.png)
- Calcium binds to bone collagen peptide by interacting with amino and carboxyl.
- Gly, Glu, Arg and Lys in this peptide were related to its ability to bind calcium.
- Calcium-binding bone collagen peptide can increase the calcium absorption.
- Calcium-binding bone collagen peptide enhanced calcium incorporation into bones.
Bone collagen peptide with high affinity to Ca was extracted from Pacific cod (Gadus macrocephalus) bone. FTIR spectra of calcium-binding bone collagen peptide showed that band at 3381Â cmâ1 shifted to 3361Â cmâ1, 1455Â cmâ1 moved to 1411Â cmâ1, and amide II became deeper valley, compared with that of bone collagen peptide. This peptide was sequenced by Q-TOF-MS and sequences of Gly-Pro-Glu-Gly, Gly-Glu-Lys, Gly-Pro-Leu-Gly and Gly-Leu-Pro-Gly appeared repeatedly in some peptides. From SEM, after chelated with calcium, the loose and porous structure turned into granular structure. From the animal experiment, Ca apparent absorption rate, Ca retention rate and femur Ca content of calcium-binding bone collagen peptide group were significantly higher than those of model and CaCO3 groups (PÂ <Â 0.05), while serum ALP was significantly lower than model group (PÂ <Â 0.05) and similar to control group. The results suggested that calcium-binding bone collagen peptide could improve bioavailability of Ca and thus prevented Ca deficiency.
Journal: Food Chemistry - Volume 221, 15 April 2017, Pages 373-378