کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5133830 1492064 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
LC-ESI-QTOF-MS identification of novel antioxidant peptides obtained by enzymatic and microbial hydrolysis of vegetable proteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
LC-ESI-QTOF-MS identification of novel antioxidant peptides obtained by enzymatic and microbial hydrolysis of vegetable proteins
چکیده انگلیسی


- Wheat, emmer, lupine, pea flour were hydrolyzed by enzymes and lactic acid bacteria.
- Fractions of hydrolysates enhanced the antioxidant activity of the substrates.
- 75 vegetable antioxidant peptides were identified by LC-ESI-QTOF-MS.
- 13 peptides were selected, synthesized and analyzed for antioxidant properties.
- 4 peptides were able to scavenge common radicals and to inhibit lipid peroxidation.

Bioactive antioxidant peptides are more and more attracting the attention of food manufacturers for their potential to transform food in functional food, able to prevent a variety of chronic diseases associated with oxidative stress. In the present study proteins extracted from different vegetable sources (KAMUT® khorasan wheat, emmer, lupine and pea) were hydrolyzed with commercial enzymes and Lactobacillus spp. strains. Hydrolysates were separated by size exclusion chromatography and purified fractions were analyzed for their antioxidant activity. Peptides from the fractions with the highest activity were identified by nanoLC-ESI-QTOF-MS and thirteen peptides were selected for synthesis on the basis of their sequence. Four peptides (VLPPQQQY, TVTSLDLPVLRW, VTSLDLPVLRW, FVPY) were found able to scavenge superoxide anion and hydroxyl radicals, organic nitro-radicals (ABTS, DPPH) and to inhibit lipid peroxidation. The impact of this work is targeted to add hydrolysed vegetable proteins to reformulated functional food or to produce health-promoting ingredients and nutraceuticals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 228, 1 August 2017, Pages 186-196
نویسندگان
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