کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5134120 1492073 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Combination of micelle collapse and field-amplified sample stacking in capillary electrophoresis for determination of trimethoprim and sulfamethoxazole in animal-originated foodstuffs
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Combination of micelle collapse and field-amplified sample stacking in capillary electrophoresis for determination of trimethoprim and sulfamethoxazole in animal-originated foodstuffs
چکیده انگلیسی


- A combination of two-state mechanism for trimethoprim and sulfamethoxazole determination was proposed.
- The sensitivity could be improved 301 and 329 times for SMZ and TMP, respectively.
- The limits of detection (LODs) were 8.5 ng/mL for TMP and 7.7 ng/mL for SMZ.
- The LODs were lower than the maximum residue limits in foods of animal origin.

An on-line preconcentration method combining micelle to solvent stacking (MSS) with field-amplified sample stacking (FASS) was employed for the analysis of trimethoprim (TMP) and sulfamethoxazole (SMZ) by capillary zone electrophoresis (CZE). The optimized experimental conditions were as followings: (1) sample matrix, 10.0 mM SDS-5% (v/v) methanol; (2) trapping solution (TS), 35 mM H3PO4-60% acetonitrile (CH3CN); (3) running buffer, 30 mM Na2HPO4 (pH = 7.3); (4) sample solution volume, 168 nL; TS volume, 168 nL; and (5) 9 kV voltage, 214 nm UV detection. Under the optimized conditions, the limits of detection (LODs) for SMZ and TMP were 7.7 and 8.5 ng/mL, and they were 301 and 329 times better compared to a typical injection, respectively. The contents of TMP and SMZ in animal foodstuffs such as dairy products, eggs and honey were analyzed, too. Recoveries of 80-104% were acquired with relative standard deviations of 0.5-5.4%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 219, 15 March 2017, Pages 7-12
نویسندگان
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