کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5136185 1494002 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous quantification six active compounds in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous quantification six active compounds in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang
چکیده انگلیسی


- Developing a UPLC-QqQ-MS/MS method to simultaneously determine five ginsenosides and muscone in rat plasma within 3.9 min.
- Pharmacokinetics profiles of six active compounds of Pien-Tze-Huang (PZH) after oral administration were obtained.
- It is the first time to report the pharmacokinetics of PZH.

Pien-Tze-Huang (PZH) is a popular traditional Chinese medicine (TCM) formula in China, but its pharmacokinetics has not been investigated yet. To better study the pharmacokinetic behaviors of PZH, an optimal ultra-performance liquid chromatography with triple quadrupole mass spectrometry (UPLC-MS/MS) method was developed for rapid quantification of six compounds (notoginsenoside R1, ginsenosides Re, Rg1, Rb1, Rd, and muscone) in rat plasma after oral administration of PZH. All analytes were extracted by protein precipitation with acetonitrile and separated on a Waters Acquity Cortecs C18 column within 3.9 min, and detected by multiple-reaction monitoring in positive ion mode. This proposed method exhibited good linearity (r ≥ 0.9932) with a lower quantification limits of 0.558-1.566 ng/mL for all analytes. The intra- and inter-day precisions were within 8.24%, and the accuracy was within −10.05 to 9.87% for each analyte. The extraction recovery for each analyte ranged from 80.02 to 96.12%. This UPLC-MS/MS method was successfully applied to the pharmacokinetic study for PZH in rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1061–1062, 1 September 2017, Pages 314-321
نویسندگان
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