کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5136314 1494008 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Centrifugal partition chromatography in the isolation of minor ecdysteroids from Cyanotis arachnoidea
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Centrifugal partition chromatography in the isolation of minor ecdysteroids from Cyanotis arachnoidea
چکیده انگلیسی


- Cyanotis arachnoidea extracts are worldwide commercialized as food supplements.
- Minor phytoecdysteroids' efficacy and safety is practically non-investigated.
- CPC method was developed for ecdysteroid isolation from a commercial extract.
- The separation was fast, efficient and reproducible.
- The yield and purity allow biological studies on the isolated compounds.

Phytoecdysteroids are known for their various beneficial bioactivities in mammals including a non-hormonal anabolic and adaptogenic effect. Cyanotis arachnoidea extracts are extensively utilized worldwide as ecdysteroid-rich materials for various purposes, e.g. food supplementation, use in agriculture and aquaculture, etc. Preparative chromatography of ecdysteroids requires extensive use of methods of different selectivity, and only a very limited number of papers are available on related application of modern liquid-liquid chromatographic techniques.In this work, a centrifugal partition chromatography (CPC) method was developed for the isolation of two minor ecdysteroids, dacryhainansterone and calonysterone, from a pre-purified commercial extract of Cyanotis arachnoidea. The biphasic solvent system was optimized by HPLC, and was composed of n-hexane - ethyl acetate - methanol - water (1:5:1:5, v/v/v/v). The isolated dacryhainansterone and calonysterone represented 99.1% and 99.7% purity, respectively.Calonysterone exerts a stronger effect on the protein kinase B (Akt) phosphorylation in mammalian skeletal muscle cells than the abundant 20-hydroxyecdysone, while no related data are available on dacryhainansterone. Despite their presence in food supplements, neither compound has appropriately been assessed for safety and efficacy. The reported method allows the gram scale isolation of these compounds, opening ways to their in-depth pharmacological investigation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1054, 1 June 2017, Pages 44-49
نویسندگان
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