کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136539 | 1494016 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Development and validation of LC-MS/MS method to identify and quantitate penicillin G and two of its metabolites, penillic acid and penilloic acid, in citrus fruit.
- Improvement of a previous method by incorporating isotopically labeled internal standards, namely, penillic acid-D5, and penilloic acid-D5.
- Method enhancement and precision of measurements.
Penicillin G has been applied to citrus trees as a potential treatment in the fight against Huanglongbing (HLB). Here, we have developed and validated a method to identify and quantitate penicillin G and two of its metabolites, penillic acid and penilloic acid, in citrus fruit using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). This method improves upon a previous method by incorporating isotopically labeled internal standards, namely, penillic acid-D5, and penilloic acid-D5. These standards greatly enhanced the accuracy and precision of our measurements by compensating for recovery losses, degradation, and matrix effects. When 2Â g of citrus fruit sample is extracted, the limits of detection (LOD) were determined to be 0.1Â ng/g for penicillin G and penilloic acid, and 0.25Â ng/g for penillic acid. At fortification levels of 0.1, 0.25, 1, and 10Â ng/g, absolute recoveries for penillic and penilloic acids were generally between 50-70%. Recoveries corrected with the isotopically labeled standards were approximately 90-110%. This method will be useful for the identification and quantitation of drug residues and their degradation products using isotopically labeled standards and UHPLC-MS/MS.
Journal: Journal of Chromatography B - Volumes 1044â1045, 15 February 2017, Pages 87-94