کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136594 | 1494019 | 2017 | 6 صفحه PDF | دانلود رایگان |
- Extraction of suvorexant (Belsomra®) from urine using liquid-liquid extraction and gas chromatography/mass spectrometry (GC/MS).
- Validation of analytical method in accordance with recommended standards in forensic toxicology.
- First report to describe the quantification of suvorexant in urine by GC/MS.
Suvorexant (Belsomra®) is a novel sedative hypnotic drug that is prescribed to promote sleep in patients with insomnia. It is the first of a new class of drugs classified as dual orexin receptor antagonists (DORAs). Sedative hypnotics with central nervous system depressant effects feature prominently in forensic toxicology investigations. For this reason, a new analytical method was developed to identify suvorexant in urine using liquid-liquid extraction (LLE) and gas chromatography/mass spectrometry (GC/MS). Due to the absence of a commercially available isotopically labeled internal standard, estazolam-D5 was used due to its azepine, triazole and chlorinated functionality. The limit of detection and limit of quantitation was 10Â ng/mL and the linear range of the assay was 10-1000Â ng/mL. Accuracy and precision (%CV) were 98-101% andâ<11% at 30, 250 and 800Â ng/mL. Interferences from matrix and fifty common drugs were not present and processed samples were stable for 24Â h at room temperature. Suvorexant is a new drug of significant forensic interest due to its hypnotic and central nervous system depressant effects. The absence of commercially available metabolites and its chromatographic properties present some challenges in terms of identification. Nevertheless, a robust, reliable and sensitive assay was developed to identify suvorexant using GC/MS analysis.
Journal: Journal of Chromatography B - Volume 1040, 1 January 2017, Pages 289-294