Rapid modulation of lipid metabolism in C57BL/6J mice induced by eicosapentaenoic acid-enriched phospholipid from Cucumaria frondosa

- This study evaluates the time-dependent effects of EPA-PL from Cucumaria frondosa on lipid metabolism.
- EPA-PL strongly suppresses the hepatic TG accumulation (3 days) and the white adipose tissue mass (7 days).
- The mechanism of EPA-PL relies on upregulation of the β-oxidation in the liver and WAT.
- EPA-PL group remains higher hepatic DHA concentration during reversal.

Recent study demonstrated that dietary eicosapentaenoic acid (EPA) could alleviate metabolism disorders. However, the onset time of EPA-enriched phospholipid (EPA-PL) on lipid metabolism was rarely been reported. The aim of the present study was to evaluate the time-dependent changes in lipid profile and genes of lipometabolism induced by EPA-PL from Cucumaria frondosa. Male C57BL/6J mice were fed a standard diet with or without EPA-PL for 3, 7, or 14 days. Additionally, we examined gene expression 1 week after removing EPA-PL from the diet. Our results indicated that 3-day EPA-PL supplementation significantly increased the expression of genes related to β-oxidation (UCP2, Ehhadh, Acaa1) in the liver and epididymal adipose tissue, whereas no differences were observed in the mRNA expression of selected genes between groups 1 week after EPA-PL removal. It is concluded that EPA-PL served as a rapid regulator of fat burning and could be a functional food for obesity disorders.