A comparison of PC oxidation products as detected by MALDI-TOF and ESI-IT mass spectrometry

We will show that chain-shortened products such as aldehydes and carboxylic acids (generated by cleavage at the double bond position) can be easily detected by both ionization methods: without the need of any derivatization. However, primary oxidation products such as hydroperoxides can be predominantly detected by ESI MS while MALDI-TOF MS detects secondary oxidation products derived thereof more sensitively. Potential reasons for these differences will be discussed and put in the context of biological mixture analysis.