کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5143290 1496472 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative microscopy based on single-molecule fluorescence
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Quantitative microscopy based on single-molecule fluorescence
چکیده انگلیسی
Quantitative microscopy is needed to understand reactions or phenomena carried out by biological molecules such as enzymes, receptors, and membrane-localized proteins. Counting the biomolecules of interest in single organelles or cellular compartments is critical in these approaches. In this brief perspective, we focus on the development of quantitative fluorescence microscopies that measure the precise copy numbers of proteins in cellular organelles or purified samples. We introduce recent improvements in quantitative microscopies to overcome undercounting or overcounting errors in certain conditions. We conclude by discussing biological applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 39, August 2017, Pages 64-73
نویسندگان
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