کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5146264 | 1497345 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production
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کلمات کلیدی
NGSRuBisCOqRT-PCRNTRHUPDMSO - DMSOPurple nonsulfur bacteria - باکتری های نئون سولفوریک بنفشBiohydrogen - بیوهیدروژنNext generation sequencing - توالی نسل بعدیDimethyl sulfoxide reductase - دی متیل سولفوکسید ردوکتازDimethyl sulfoxide - دیمتیل سولفواکسیدNitrogen - نیتروژنHydrogen - هیدروژن Uptake hydrogenase - هیدروژناز را جذب کنیدquantitative reverse transcription polymerase chain reaction - واکنش زنجیره ای پلی مراز رونویسی معکوسBacterial genomics - ژنومیک باکتریایی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production](/preview/png/5146264.png)
چکیده انگلیسی
To overcome efficiency losses due to overheating in outdoor photobioreactors for microbial hydrogen production, heat-resistant microorganisms are desirable. In this study, the comparative whole genome sequencing analyses of two previously obtained Rhodobacter capsulatus heat-resistant mutants; A52 and B41, with modified hydrogen production capacities, have been performed to identify mutations related to hydrogen production. In comparison with the reference strain DSM1710, the genomes of the mutants A52 and B41 contained 2137 and 2253 mutations, respectively. In the mutant B41 genome, mutations were characterized within the nifD, nifJ, glnD, nifB1, ccpA, hupD, dmsA, and cbbR1 genes. On the other hand; in the A52 mutant, mutations were characterized in the nifB2, rnfF, nifJ, cbbO, anfH, amt, moeA, and hupD genes. The effects of nitrogen metabolism and redox-related mutations were tested by quantitative reverse transcription PCR. The most promising mutation was found on the glnD gene of B41 strain with higher hydrogen production capacity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 42, Issue 32, 10 August 2017, Pages 20529-20539
Journal: International Journal of Hydrogen Energy - Volume 42, Issue 32, 10 August 2017, Pages 20529-20539
نویسندگان
Abdulmecit Gökçe, Yavuz Ãztürk, Zeynep Petek Ãakar,