Effect of concentration on biohydrogen production in a continuous stirred bioreactor using biofilm induced packed-carrier

The optimum substrate concentration of 20 g total sugar/L medium was obtained at HPR of 20.37 L H2/L.d, yield of 1.41 mol H2/mol hexose and substrate utilization of 91.71%, respectively, by using packed-filter M 190. The biohydrogen production rate was 34% higher (HPR of 27.27 ± 1.05) in M365 carrier than in M190 (HPR of 20.37 ± 0.86) when HRT was 4 h. Finally, using commercial Flex-matal as a packed-filter in the bioreactor for enhancement biohydrogen production could be an easier and cheaper operation strategy.