Improving and optimizing protein concentration yield from homogenized baker's yeast at different ratios of buffer solution

In this study, the mechanical pre-treatment of yeast substrate was conducted through its homogenization in the high-pressure homogenizer (HPH). With the operating conditions of temperature, pressure and number of passes (cycles) ranging from (30-50 °C), (30-90 MPa) and (1-5) respectively, this was ensured that the HPH machine maximum pressure of 100 MPa was not exceeded. The homogenized yeast was diluted in three levels of dilutions with buffer solutions; denoted as solution C in the ratio of; 10:90, 20:80; and 30:70 respectively. The resultant protein concentration yield so obtained was analyzed and optimized through using the Design Expert V.8 in the comparison of the three samples in the ratios given with the 3 input factors of pressure, temperature and number of cycles estimated within the considered limits.