کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5147650 | 1497388 | 2016 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improving and optimizing protein concentration yield from homogenized baker's yeast at different ratios of buffer solution
ترجمه فارسی عنوان
بهبود و بهینه سازی عملکرد غلظت پروتئین از مخمر نانوایی همگن شده با نسبت های مختلف محلول بافر
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
چکیده انگلیسی
In this study, the mechanical pre-treatment of yeast substrate was conducted through its homogenization in the high-pressure homogenizer (HPH). With the operating conditions of temperature, pressure and number of passes (cycles) ranging from (30-50 °C), (30-90 MPa) and (1-5) respectively, this was ensured that the HPH machine maximum pressure of 100 MPa was not exceeded. The homogenized yeast was diluted in three levels of dilutions with buffer solutions; denoted as solution C in the ratio of; 10:90, 20:80; and 30:70 respectively. The resultant protein concentration yield so obtained was analyzed and optimized through using the Design Expert V.8 in the comparison of the three samples in the ratios given with the 3 input factors of pressure, temperature and number of cycles estimated within the considered limits.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 41, Issue 37, 5 October 2016, Pages 16415-16427
Journal: International Journal of Hydrogen Energy - Volume 41, Issue 37, 5 October 2016, Pages 16415-16427
نویسندگان
Leonard E.N. Ekpeni, Khaled Y. Benyounis, Joseph Stokes, Abdul G. Olabi,