کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5148458 | 1497371 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improvement of fermentative hydrogen production using genetically modified Enterobacter aerogenes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The [NiFe]-hydrogenase 3 of Enterobacter aerogenes contains a large subunit of 60Â kDa (HycE) and a small subunit of 30Â kDa (HycG). The gene of HycE and the gene of HycG were overexpressed in E. aerogenes separately by using the pGEX-4T-2-cat vector, to obtain two recombinant strains: E. aerogenes/HycE and E. aerogenes/HycG. The hydrogen yields were significantly enhanced by the recombinant strains. The hydrogenase activities of the recombinant strains increased by 124% (E. aerogenes/HycE) and 67% (E. aerogenes/HycG) compared with that of the wild strain. The hydrogen yields of the recombinant strains from initial glucose increased by 86.2% to 2.16Â mol H2/mol (E. aerogenes/HycE) and by 69.8% to 1.97Â mol H2/mol (E. aerogenes/HycG). The recombinant strains produced more acetate and butyrate than the wild strain, and this finding corresponded to the metabolism of NADH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 42, Issue 6, 9 February 2017, Pages 3676-3681
Journal: International Journal of Hydrogen Energy - Volume 42, Issue 6, 9 February 2017, Pages 3676-3681
نویسندگان
Jinfang Zhao, Wenlu Song, Jun Cheng, Min Liu, Chuanxi Zhang, Kefa Cen,