Aspartate aminotransferase is potently inhibited by copper complexes: Exploring copper complex-binding proteome

The 97 putative Cu-binding proteins from primary rat hepatocytes were isolated and identified. Five putative Cu-binding proteins were further purified and the interaction between them and copper complexes was investigated. Complexes 6 and 7 potently inhibited aspartate aminotransferase (AST) and 6 entered into HepG2 cells and inhibited intracellular AST activity.82