Protein oxidation involved in Cys-Tyr post-translational modification

Native fluorescence allows quantitative determination of cysteine-tyrosine crosslink (Cys-Tyr) formation and with high selectivity in the presence of dityrosine. Mutagenic analysis of Cys-Tyr formation in an orphan protein (BF4112) with a mononuclear copper center showed protein oxidation to dityrosine is a significant side reaction opposed to galactose oxidase.197