Anti-inflammatory and anti-angiogenic activities of a purified polysaccharide from flesh of Cipangopaludina chinensis

The purpose of this paper was to investigate the anti-inflammatory and anti-angiogenic activities of a purified polysaccharide (CCPSn) from flesh of Cipangopaludina chinensis in lipopolysaccharide (LPS) activated RAW264.7 macrophages and human umbilical vein endothelial cells (HUVECs), respectively. Anti-inflammatory results showed that CCPSn not only greatly decreased the pro-/anti-inflammatory cytokine secretion ratios, including TNF-α/IL-10, IL-6/IL-10 and IL-1β/IL-10, but also reduced release levels of nitric oxide (NO) and prostaglandin E2 (PGE2), and suppressed expressions of cyclooxygenase2 (COX2) and inducible nitric oxide synthase (iNOS). The results from anti-angiogenic activities revealed that proliferation, migration, tube formation and vascular endothelial growth factor (VEGF) of HUVECs were significantly inhibited by CCPSn treatment. Even more remarkable was the fact that anti-inflammatory and anti-angiogenic activities of CCPSn exhibited a clear dose-response manner, and these activities were irrelevant to the cytotoxicities of CCPSn to RAW264.7 macrophages and HUVECs. These results indicated that CCPSn possessed strong anti-inflammatory and anti-angiogenic activities.